by Kip AE, Rosing H, Hillebrand MJ, Blesson S, Mengesha B, Diro E, Hailu A, Schellens JH, Beijnen JH, Dorlo TP. Antimicrobial Agents and Chemotherapy. 2016 Jan 19. pii: AAC.02976-15.
Summary: To facilitate future pharmacokinetic studies of combination treatments against leishmaniasis in remote endemic regions, a simple and cheap sampling methodology was required for miltefosine quantification. The aim of this study was to validate a liquid chromatography-tandem mass spectrometry method to quantify miltefosine in dried blood spots, and to validate its use in Ethiopian visceral leishmaniasis patients. The method was demonstrated to be a valid and practical alternative to testing blood sampled from veins, and can be applied in future miltefosine pharmacokinetic studies in leishmaniasis patients.